Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. EpCAM and CD45 expression, karyotyping, and 6-thioguanine effects on 4T1 cells and sublines.
  • Fig. S2. Quantification of EMT markers, cell growth, 2D and 3D colony formation, cell adhesion, migration, and invasion of 4T1 cells and sublines CTC1 and DTC1.
  • Fig. S3. Analysis of tumor growth, metastases formation, EpCAM and vimentin expression, ex vivo cultures, and GO terms and genes associated with DNA breakpoints in 4T1 cells and sublines CTC1 and DTC1.
  • Fig. S4. Analysis of EMT scores and markers in 4T1 cells, E/m- and M/e-type sublines.
  • Fig. S5. Analysis of cell proliferation, adhesion, sensitivity to chemotherapy, and metastasis formation in 4T1 cells, E/m-, M/e-, and M-type sublines.
  • Fig. S6. Analysis of morphology, EMT scores, and EpCAM expression of 4T1-, CTC1-, and DTC1-derived single cell clones, and DTC1-derived CTC sublines.
  • Fig. S7. EpCAM expression in 4T1- and CTC1-derived primary tumors and metastases, and in primary tumors, lymph node and distant metastases of clinical samples of MBC.
  • Fig. S8. Patients’ characteristics and ploidy and cell size of CTCs and DTCs from MBC patients.
  • Fig. S9. Copy number variations in EpCAM+ and EpCAM CTCs from MBC patients.
  • Table S1. Enrichment analysis of GO biological process terms of CNVs from EpCAM+ and EpCAM CTCs.

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