Science Advances

Supplementary Materials

The PDF file includes:

  • General materials and methods for organic synthesis
  • Synthesis of N-Fmoc–protected TFMD-Phe
  • General materials and methods for Aβ16–22 solid-phase peptide synthesis
  • General materials and methods for HPLC purification
  • Analytical MS and HPLC data for synthetic peptides
  • General materials and methods for recombinant peptide synthesis
  • Additional characterization and analyses
  • CCS analysis of Aβ40 in the presence and absence of Aβ16–22
  • Scheme S1. Synthesis of TFMD-Phe.
  • Fig. S1. HRMS and analytical HPLC traces of Aβ16–22 and its variants.
  • Fig. S2. SEC trace of Aβ40 indicates that there is a single peak, and ESI-IMS-MS indicates that in the gas phase Aβ40 is largely monomeric.
  • Fig. S3. Supplementary ThT data.
  • Fig. S4. Supplementary negative-stain TEM images.
  • Fig. S5. Analysis of the CCS values for Aβ40 in the absence or presence of Aβ16–22 over different IMS experiments.
  • Fig. S6. PIC analysis of 1:1 Aβ*16–22/Aβ40 at 5 min and 24 hours.
  • Fig. S7. Plot of the average number of hydrogen bonding and side chain–side chain.
  • Table S1. The expected and observed m/z values for monomeric and oligomeric Aβ40 in isolation and in the presence of a 1:1 ratio of Aβ16–22.
  • Table S2. Assignments of each of the major peaks observed in fig. S6A.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (.pdb format). MD snapshots as pdb files Fig. 1 t = 0.
  • Data file S2 (.pdb format). MD snapshots as pdb files Fig. 1 t = 104.
  • Data file S3 (.pdb format). MD snapshots as pdb files Fig. 1 t = 230.
  • Data file S4 (.pdb format). MD snapshots as pdb files Fig. 1 t = 621.
  • Data file S5 (.pdb format). MD snapshots as pdb files Fig. 6 t = 0.29.
  • Data file S6 (.pdb format). MD snapshots as pdb files Fig. 6 t = 1.16.
  • Data file S7 (.pdb format). MD snapshots as pdb files Fig. 6 t = 1.93.
  • Data file S8 (.pdb format). MD snapshots as pdb files Fig. 6 t = 7.7.
  • Data file S9 (.pdb format). MD snapshots as pdb files Fig. 6 t = 29.
  • Data file S10 (.pdb format). MD snapshots as pdb files Fig. 6 t = 77.7.

Files in this Data Supplement: