Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Generation of stable, inducible cell lines expressing E2F1 methylation site mutants.
  • Fig. S2. Additional analysis of RNA-seq and rMATS datasets.
  • Fig. S3. GO biological process enrichment analysis on spliced E2F1 target genes from the RNA-seq data.
  • Fig. S4. Additional analysis of E2F1 RIP-seq datasets.
  • Fig. S5. Expression of E2F1 correlates with PRMT5 and MECOM V7 transcript expression in human cancer.
  • Legends for tables S1 to S6

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of up- and down-regulated E2F1 target genes identified from the RNA-seq analysis for each cell line, corresponding to Fig. 1B.
  • Table S2 (Microsoft Excel format). List of alternative splicing events in E2F1 target genes identified in the RNA-seq rMATS analysis corresponding to the heatmap (Fig. 2A).
  • Table S3 (Microsoft Excel format). Differential expression of genes associated with RNA splicing, taken from the RNA-seq dataset (Fig. 1B).
  • Table S4 (Microsoft Excel format). List of RNAs identified in the anti-E2F1 RIP-seq analysis (Fig. 4).
  • Table S5 (Microsoft Excel format). List of overlapping E2F target genes between RIP-seq dataset (Fig. 4) and splicing analysis (Fig. 2A).
  • Table S6 (Microsoft Excel format). List of E2F1 RIP-seq reads that span exon junctions.

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