Science Advances

Supplementary Materials

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  • Fig. S1. Quantitative demonstration of various fractions of m6A-containing oligo mixed with unmethylated oligo digested by ChpBK.
  • Fig. S2. FTO demethylate assay.
  • Fig. S3. The base composition of mRNA products cleaved by MazF.
  • Fig. S4. The single-site validation for 18S rRNA control site and m6A site.
  • Fig. S5. The single-site validation of eight m6A sites and one unmethylated site.
  • Fig. S6. Quantitative PCR results of six m6A sites.
  • Fig. S7. Metagene plots of m6A in mouse heart and mouse testis.
  • Fig. S8. Conservation of m6A in mammalian kidney.
  • Fig. S9. Conservation of m6A in mammalian liver.
  • Fig. S10. Gene expression and the expected number of shared m6A-modified genes.
  • Fig. S11. Evolutionary conservation between methylated and unmethylated A sites in the same genes of brain tissue.
  • Fig. S12. Evolutionary conservation between methylated and unmethylated A sites in the same genes of kidney tissue.
  • Fig. S13. Evolutionary conservation between methylated and unmethylated A sites in the same genes of liver tissue.
  • Fig. S14. Conservation scores for brain.
  • Fig. S15. Validation for the methylation sensitivity of mutated MazF-K56A.
  • Table S1. Basic information of sequencing data for human HEK293T cell line.
  • Table S2. Designed probes and universal primers for T3 ligase–based validation.
  • Table S3. Designed primers for Quantitative PCR validation.
  • Table S4. Basic information of sequencing data for mammalian tissues.
  • Table S5. m6A sites identified by m6A-REF-seq for mammalian tissues.
  • Table S6. Shared m6A-modified genes and m6A sites between pairwise species and the significance.

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