Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Injury causes persistent allodynia.
  • Fig. S2. ppk+ sensory neuron projections to the VNC and brain.
  • Fig. S3. Electrophysiological properties of the nociceptive escape circuit.
  • Fig. S4. Peripheral injury causes a loss of GABAergic interneurons.
  • Fig. S5. Peripheral injury causes reduction in GABA in the VNC but not the brain.
  • Fig. S6. Knockdown of Grd, GABA-B-R1, or GABA-B-R3 does not cause allodynia in uninjured flies, cholinergic output from ppk+ neurons mediates acute nociception behavior, and Twist is important for GABA loss after injury and mediates heat allodynia.
  • Table S1. List of antibodies used in immunochemical experiments.
  • Legends for movies S1 and S2
  • Legend for table S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Uninjured wild-type animals exhibit escape behavior in response to temperatures of ≥42°C.
  • Movie S2 (.mp4 format). Peripheral injury causes increase in thermal allodynia in wild-type flies.
  • Table S2 (Microsoft Excel format). Detailed data of behavioral, immunochemical, and electrophysiological experiments.

Files in this Data Supplement: