Science Advances

Supplementary Materials

This PDF file includes:

  • Supplemental Materials and Methods
  • Experimental Protocols
  • Supplementary Note A. Development of the conditions for the dynamic reaction network by characterization of the individual enzyme reactions
  • Supplementary Note B. Routine of GE analysis: From the agarose gel to an average chain length
  • Supplementary Note C. ATP-fueled transient, dynamic steady-state DNA polymerization system
  • Supplementary Note D. DySS and molecular exchange in ATP-fueled dissociative dynamic covalent DNA systems
  • Table S1. Oligonucleotide sequences.
  • Fig. S1. Hybridization of the self-complementary ends of the DNA monomer strands M1 in dependence of temperature and ligation reaction catalyzed by T4 DNA ligase.
  • Fig. S2. Ligation kinetics of the DNA chain growth as a function of T4 DNA ligase concentration.
  • Fig. S3. Ligation kinetics of the DNA chain growth as a function of ATP concentration.
  • Fig. S4. Time-dependent T4 DNA ligase catalyzed ligation reaction.
  • Fig. S5. Restriction kinetics of the DNA chain cleavage as a function of BamHI concentration.
  • Fig. S6. Routine for analysis of GE data: From the agarose GE to an average DNA chain length bp¯w.
  • Fig. S7. Control of dispersity in the DySS DNA polymerization system.
  • Fig. S8. Refueling experiments of the transient DySS DNA polymerization system.
  • Fig. S9. Average chain length in the transient DySS DNA polymerization system in dependence of the concentration of the DNA monomer M1.
  • Fig. S10. Characterization of the FRET duplex F and its cleaved and religated DNA fragments as used for in situ modulation of the DySS.
  • Fig. S11. ATP-dependent temporal control of the dynamic DNA bond with transient DySS FRET duplex formation.
  • References (40, 41)

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