Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Freshly collected specimens of B. solaris.
  • Fig. S2. Freshly collected specimens of P. paranygulgus.
  • Fig. S3. LM and CLSM micrographs of P. paranygulgus.
  • Fig. S4. TEM micrographs of B. solaris.
  • Fig. S5. Taxonomic partitioning of the transcriptome of B. solaris with BlobTools.
  • Fig. S6. Bayesian majority-rule consensus tree of the partial psbA gene recovered from the transcriptome of B. solaris.
  • Fig. S7. Bayesian majority-rule consensus tree of the partial psbB gene recovered from the transcriptome of B. solaris.
  • Fig. S8. Bayesian majority-rule consensus tree of the partial psaB gene recovered from the transcriptome of B. solaris.
  • Fig. S9. Bayesian majority-rule consensus tree of the partial atpA gene recovered from the transcriptome of B. solaris.
  • Fig. S10. Bayesian majority-rule consensus tree of the partial rbcL gene recovered from the transcriptome of B. solaris.
  • Fig. S11. Bayesian majority-rule consensus tree based on an alignment of rbcL sequences of representative pennate diatoms, PCR-amplified rbcL sequences from the kleptoplasts of different specimens of B. solaris and P. paranygulgus, and rbcL transcripts recovered from the transcriptome of B. solaris.
  • Fig. S12. Loss of kleptoplasts in three specimens of B. solaris during the starvation experiment.
  • Fig. S13. Busco assessment results of the assembled transcriptome of B. solaris and other publicly available flatworm transcriptomes.
  • Table S1. Amplification primers and thermocycling conditions for the partial rbcL gene.
  • Table S2. Best-fit partitioning schemes and models of molecular evolution for five plastid genes and the concatenated alignment recovered in PartitionFinder v1.1.0.
  • Legends for movies S1 to S3
  • Legends for data files S1 and S2
  • References (4849)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Live adult specimen of B. solaris full of kleptoplasts squeezed under a cover slip.
  • Movie S2 (.mov format). Live adult specimen of B. solaris full of kleptoplasts squeezed under a cover slip.
  • Movie S3 (.mov format). Compilation of CLSM micrographs showing the autofluorescence of the kleptoplasts in a live juvenile specimen of P. paranygulgus.
  • Data file S1 (Microsoft Excel format). Blastp and blastx annotated transcripts of the plastid-encoded genes, psbA, psbB, atpA, psaB, and rbcL, in the transcriptome of B. solaris are almost exclusively of diatom origin.
  • Data file S2 (Microsoft Excel format). Blastp and blastx annotated transcripts of diatom origin in the transcriptome of B. solaris are all plastid-encoded.

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