Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Fine-mapped PCa risk SNPs in 12q13 region.
  • Fig. S2. SNP rs55958994 is associated with enhancer activity in cervical, liver, and breast cancer cell lines.
  • Fig. S3. CRISPR-Cas9–mediated deletion of the rs55958994-associated enhancer.
  • Fig. S4. Re-expression of target genes in enhancer-deleted PCa cells promotes cancer initiation, growth, and progression.
  • Fig. S5. Re-expression of KRT8 in enhancer-deleted PCa cells did not rescue the defect of soft agar colony formation and invasive migration ability and the decrease in CSC population.
  • Fig. S6. Validation of target gene expression in enhancer KO cells.
  • Fig. S7. 3C-PCR analysis confirms interaction of the rs55958994-associated enhancer and target gene loci.
  • Fig. S8. Comparison of transcriptomic changes following deletion of the rs55958994-associated enhancer and mutation of rs55958994.
  • Table S1. Guide RNAs for CRISPR-Cas9–mediated deletions.
  • Table S2. Primers used in qRT-PCR experiment.
  • Table S3. Primers for 3C-PCR assay.
  • Table S4. Guide RNAs for CRISPR-Cas9–mediated SNP editing.

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