Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Characterization of cell decision-making at trifurcating Ψ-like branch channels of different hydraulic resistances.
  • Fig. S2. Effects of different cytoskeletal constituents on cell decision-making in response to hydraulic resistance.
  • Fig. S3. Hydrostatic pressure induces intracellular calcium increase and a thicker cortical actin meshwork.
  • Fig. S4. High hydraulic resistance results in larger changes of membrane curvature.
  • Fig. S5. Variations in bleb size and cortical actin intensity reach a minimum at the decision-making time point, suggesting a physical balance between internal and external cell forces.
  • Table S1. Dimensions of the different trifurcated designs.
  • Table S2. Comparison between the experimental and theoretical probabilities of cells entering branches in two different devices.
  • Table S3. Comparison between the experimental and theoretical probabilities of cells entering branches in multiple devices.

Download PDF

Files in this Data Supplement: