Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Biochemical and functional characterization of the TRPC5 constructs.
  • Fig. S2. Flow chart for TRPC5 cryo-EM data processing.
  • Fig. S3. Pore loop structures of known TRP channels.
  • Fig. S4. Comparison of ion conducting pathways between TRPC5 and TRPC4.
  • Fig. S5. Cell surface expression levels of TRPC4 cysteine mutants and function of TRPC5 cysteine mutants.
  • Fig. S6. Gd3+ potentiation is dependent on the unliganded and partially open state of TRPC5.
  • Fig. S7. The cation binding sites.
  • Fig. S8. Lipid-channel interactions.
  • Fig. S9. Pore loop interactions and intracellular mouth of the channel pore.
  • Table S1. Cryo-EM data collection, refinement, and validation statistics.

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