Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. The origins of common subgraphical motifs shared by five Ω-family AMPs.
  • Fig. S2. Checkerboard assay to determine whether Ω76 and colistin in combination display a synergistic, additive, or antagonistic effect in vitro.
  • Fig. S3. Blood tests performed for multidose Ω76-treated mice in comparison to untreated control mice.
  • Fig. S4. Pilot experiments to determine the in vivo efficacy of Ω76 against A. baumannii (P1270) using a BALB/c mouse peritoneal model of infection.
  • Fig. S5. Independent replication experiment to confirm Ω76 activity against A. baumannii (P1270) in BALB/c mice, performed by A.D.
  • Fig. S6. SEM experiments performed for A. baumannii (B4505).
  • Fig. S7. SEM experiments performed for A. baumannii (B4505) protoplasts.
  • Fig. S8. SEM experiments performed for E. coli (K12 MG1655).
  • Fig. S9. SEM experiments performed for S. flexneri (MTCC1457).
  • Fig. S10. The classification of genes into GO terms after enrichment analysis.
  • Fig. S11. Ramachandran plot analysis of the best 30 structures of Ω76.
  • Fig. S12. The electrostatic surface potential and hydrophobic moment of Ω76.
  • Fig. S13. An illustration for all the steps required to determine the hemolytic activity of an AMP using human blood.
  • Fig. S14. Titration to determine the dose of A. baumannii (P1270) to be used for later efficacy experiments.
  • Fig. S15. The protocols used for generating time-kill curves are illustrated in detail.
  • Fig. S16. The protocol used for the 32PO34 leakage radioassay is illustrated in detail.
  • Table S1. A detailed tabulation depicting the origin of common subgraphical motifs shared by five Ω-family AMPs.
  • Table S2. MIC values expressed in micrograms per liter for 30 cultures tested against five designed peptides.
  • Table S3. MBC values expressed in micrograms per liter for Gram-positive cultures tested against two designed peptides displaying the highest I_scores (Ω17 and Ω76).
  • Table S4. MBC values expressed in micrograms per liter for Gram-negative cultures tested against two designed peptides displaying the highest I_scores (Ω17 and Ω76).
  • Table S5. MBC values expressed in micrograms per liter for seven clinical isolates of A. baumannii.
  • Table S6. 16S rRNA gene sequencing data for A. baumannii (P1270), confirming the genus and species.
  • Table S7. List of significantly up-regulated genes for A. baumannii (P1270) exposed to 0.1×, 0.25×, and 0.5× MBC concentrations of Ω76.
  • Table S8. List of significantly down-regulated genes for A. baumannii (P1270) exposed to 0.1×, 0.25×, and 0.5× MBC concentrations of Ω76.
  • Table S9. NMR restraints and structure evaluation statistics for Ω76 (30 structures).

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Other Supplementary Material for this manuscript includes the following:

  • Text S1 (.pdb format). Coordinates of the 30 lowest-scoring NMR models of Ω76.
  • Dataset S1 (Microsoft Excel format). Raw data for all figures.
  • Dataset S2 (.zip format). All original images acquired during confocal microscopy experiments are provided.
  • Dataset S3 (.zip format). Raw NOESY and TOCSY spectra.
  • Dataset S4 (.csv format). A. baumannii gene to GO term mappings.
  • Protocol S1 (.zip format). HELIGRAPHER.

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