Science Advances

Supplementary Materials

The PDF file includes:

  • Table S1. Data collection and refinement statistics for Twitch-2B.
  • Table S2. Data collection and refinement statistics for Twitch-6.
  • Fig. S1. Crystal packing of Twitch-2B.
  • Fig. S2. The SAXS data of Twitch-2B show monomeric state in solution.
  • Fig. S3. Donor dequenching of the calcium-bound Twitch proteins.
  • Fig. S4. Emission spectrum (excitation, 432 nm) of Twitch-6 (Twitch-2B N532F) in calcium-free state (black trace) and at calcium saturation (red trace).
  • Fig. S5. Experimental fluorescence absorbance and emission spectra of the isolated cpVenus and mCerulean3, respectively.
  • Fig. S6. Pulse-sequence scheme for the J-modulated HMQC-TROSY experiment used to determine 1JHC couplings (Ca-loaded samples) and the corresponding 1DHC (Dy-loaded samples).
  • Fig. S7. Characterization of dynamics in Twitch-2B and Twitch-6 by paramagnetic NMR.
  • Fig. S8. Example of intensity peak modulation of the J-modulated HMQC-TROSY experiment from Twitch-2B acquired at 1.1 GHz.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1. Structure_Based_FRET_Twitch-2B.xlsx (Excel file).
  • Data file S2. (Python script).

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