Science Advances

Supplementary Materials

The PDF file includes:

  • Supplementary Text
  • Fig. S1. Microfabrication, optimization, and characterization for FemDA device.
  • Fig. S2. Measurement of the dynamic surface tension of the flush oil.
  • Fig. S3. Bulk-phase CFPS.
  • Fig. S4. Histogram of fluorescence intensities of droplets from various protein samples on FemDA.
  • Fig. S5. CFPS by E. coli cell lysates.
  • Fig. S6. Verification and establishment of amplification protocol for one DNA molecule.
  • Fig. S7. Analyses of the E. coli ALP mutants.
  • Fig. S8. Changes of interaction energy between the E. coli ALP 109th amino acid residue and its surrounding amino acid residues.
  • Fig. S9. Fluorescent fusion proteins to assess target protein yield.
  • Table S1. Theoretical maximum size of gene library for specific settings of screening.
  • Legends for movies S1 to S5
  • References (3840)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Fluorescence recovery after photobleaching (FRAP) experiment for the confirmation of the robust droplet formation.
  • Movie S2 (.avi format). Time-course measurement for CFPS of fluorescent protein mVenus.
  • Movie S3 (.avi format). CFPS of mVenus using FemDA.
  • Movie S4 (.avi format). Time-course measurement for CFPS of ALP.
  • Movie S5 (.avi format). Nucleic acid recovery from femtoliter droplets with a microcapillary.

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