Science Advances

Supplementary Materials

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  • Fig. S1. Representative tetramer-guided epitope mapping for H1HA and surface expression of DQ molecules on HEK293 cells.
  • Fig. S2. Cross-reactivity of islet-specific T cell clones was investigated by staining of DQ8cis and DQ8trans tetramers and a representative ex vivo staining for DQ8trans/GAD65250–266 and DQ8trans/IA-2957–972 from one T1D and one healthy participant.
  • Fig. S3. DQ8cis and DQ8trans elicit T cell responses by the same antigenic motif.
  • Fig. S4. Affinity and presentation of shared epitopes.
  • Fig. S5. TCR usages for GAD65250–266-specific T cell clones and investigation of cross-reactivity of islet-specific T cell clones by different DQ restriction elements.
  • Table S1. List of common Influenza A epitopes presented by DQ2cis.
  • Table S2. Summary of ex vivo results for GAD65250–266 and IA-2957–972.
  • Table S3. List of the T cell clones, their proliferative capacity, and cytokine secretion.
  • Table S4. Comparison of functional avidity EC50 between wild-type DQ8trans and other DQ8cis/trans variants in complex with GAD65250–266.
  • Table S5. Predicted interactions between p9Arg/Arg76α with residues at pocket 9 of DQ8cis or DQ8trans.

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