Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Genomic map and annotated base pair sequence of rIgG2a constant domains.
  • Fig. S2. Sortagging of Fab′ fragments derived from CRISPR/HDR-engineered hybridomas.
  • Fig. S3. Characterization of MIH5 Fc variants hybridomas.
  • Fig. S4. Raw images of sortagging of MIH5 isotype variants.
  • Fig. S5. Murine isotype panel generation of NLDC-145 via CRISPR/HDR.
  • Fig. S6. Glycosylation profile of MIH5 mIgG2a via native mass spectrometry.
  • Fig. S7. Gating and FACS plots of isotype-dependent depletion in vivo.
  • Table S1. Fab′ donor construct for HDR.
  • Table S2. Isotype donor constructs for HDR.
  • Table S3. FcγR affinity values of MIH5 Fc variants and comparison to literature.

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