Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Representative HMO molecules analyzed in this study.
  • Fig. S2. Schematic representation of two different gene disruption methods used in this study.
  • Fig. S3. Evaluation of the HMO transporter–heterologous expression system.
  • Fig. S4. ITC and SPR analyses of SBPs of FL transporter-1 and -2.
  • Fig. S5. Structural basis of the dual recognition of 2′-FL and 3-FL by FL2-BP from B. infantis JCM 1222T.
  • Fig. S6. Phylogenetic and structural analyses revealed the signature sequences that dictate specificity difference between FL1-BP and FL2-BP homologs.
  • Fig. S7. HMO consumption analysis using samples collected from 32 mother-infant pairs.
  • Table S1. Data collection and refinement statistics for the SBP of FL transporter-2 from B. infantis JCM 1222T (FL2-BP).
  • Table S2. Polar contacts between FL2-BP and FL molecules.
  • Table S3. Primers and probes used in this study.

Download PDF

Files in this Data Supplement: