Science Advances

Supplementary Materials

This PDF file includes:

  • Table S1. Sequence of the synthesized oligonucleotides.
  • Fig. S1. Annealing and gene silencing ability of synthesized siRNA.
  • Fig. S2. Chemical structure of DEX-MAES.
  • Fig. S3. Schematic illustration of siRNA conjugation to hydrogels via Michael-addition reaction and its release.
  • Fig. S4. Effect of hydrogel concentration on the tethered siRNA release behavior and bioactivity of released siRNA.
  • Fig. S5. Lipofectamine-mediated bioactivity of released siRNA from siRNA-tethered hydrogels.
  • Fig. S6. Transfection reagent-mediated bioactivity of siRNA loaded or tethered in the hydrogels.
  • Fig. S7. Schematic illustration of siRNA conjugation to hydrogels via photoconjugation and its release.
  • Fig. S8. Additional release profiles of tethered siGFP from hydrogels with longer sampling intervals compared to Fig. 3A.
  • Fig. S9. Additional bioactivity data of released siGFP (200 and 350 nM RNA treatment).

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