Science Advances

Supplementary Materials

The PDF file includes:

  • Section S1. The FEM simulation
  • Section S2. 2D Gaussian fitting
  • Section S3. SBR evaluation
  • Table S1. 1/τon and 1/τoff of TriM-β-CD with different TriM-β-CD.
  • Table S2. 1/τon of TriM-β-CD with different KCl in cis.
  • Table S3. FWHM and SBR with different KCl.
  • Table S4. FWHM and SBR with different CaCl2.
  • Table S5. FWHM and SBR of α-HL and ClyA-RR nanopores.
  • Table S6. Nucleic acid abbreviations and sequences.
  • Table S7. Blockade level of dsDNA events.
  • Fig. S1. FEM model geometry.
  • Fig. S2. The DIB device.
  • Fig. S3. The schematic diagram of the setup.
  • Fig. S4. Cyclodextrin binding kinetics.
  • Fig. S5. Definition of signal and background during oSCR.
  • Fig. S6. Demonstration of fluorescence trace normalization.
  • Fig. S7. Event statistics derivation.
  • Fig. S8. Baseline comparison during TriM-β-CD sensing.
  • Fig. S9. FEM modeling of Fluo-8 distribution.
  • Fig. S10. FEM modeling of the osmotic flow.
  • Fig. S11. The preparation and characterization of ClyA-RR.
  • Fig. S12. Observing dsDNA events with different dsDNA concentrations.
  • Fig. S13. Statistics of dsDNA events acquired from ClyA-RR.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Simultaneous imaging of α-HL and ClyA.
  • Movie S2 (.mp4 format). dsDNA sensing by DOP.
  • Movie S3 (.mp4 format). Parallel dsDNA sensing by DOP.
  • Movie S4 (.mp4 format). A single ClyA-RR nanopore inserted in a miniaturized DIB.

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