Science Advances

Supplementary Materials

The PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. OBB displacement during SWIFT.
  • Fig. S2. Luminal channels formed after sacrificial ink printing and removal.
  • Fig. S3. Rheological characterization of tissue matrix constituents.
  • Fig. S4. Tissue viability assessment.
  • Fig. S5. Branching vascular network embedded within an EB-based tissue matrix via SWIFT.
  • Fig. S6. Embedded 3D printing of OBBs within different living matrices.
  • Fig. S7. Schematic illustrations of perfusion apparatus for SWIFT biomanufacturing and tissue maintenance.
  • Fig. S8. Viability of perfused and nonperfused EB-based tissues.
  • Fig. S9. Custom-made perfusion chip for large-scale viability assay.
  • Fig. S10. Flow cytometry data for cardiac OBBs.
  • Fig. S11. Cardiac tissue composition.
  • Fig. S12. Calcium imaging of cardiac tissue matrix.
  • Fig. S13. Characterization of cardiac tissue produced by SWIFT.
  • Fig. S14. Characterization of perfused cardiac tissue.
  • Legends for movies S1 to S8

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Nozzle translation during embedded printing within a tissue matrix composed of OBBs.
  • Movie S2 (.mp4 format). Biomanufacturing of a helical vascular feature within a tissue matrix composed of OBBs.
  • Movie S3 (.mp4 format). Biomanufacturing of a branched vascular network within a tissue matrix composed of OBBs.
  • Movie S4 (.mp4 format). Endothelialization of a vascular channel within a tissue matrix composed of OBBs.
  • Movie S5 (.mp4 format). OBBs composed of beating cardiac organoids.
  • Movie S6 (.mp4 format). Calcium imaging of cardiac OBB matrix.
  • Movie S7 (.mp4 format). Cardiac tissues with perfusable vascular channels via SWIFT.
  • Movie S8 (.mp4 format). Contractile activity of a bulk cardiac tissue (T-shaped construct) produced by SWIFT biomanufacturing.

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