Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. The placement of rhizoboxes and observed disease dynamics during the field experiment.
  • Fig. S2. Differences in the physicochemical soil properties, total bacterial abundances, and bacterial diversity between healthy and diseased plants during the field experiment.
  • Fig. S3. Relationships between physical distance and bacterial phylogenetic distance (unweighted UniFrac distance) between rhizoboxes within three replicated plots.
  • Fig. S4. Differences in the abundances of bacterial phyla in the initial soils (week 0) that later became associated with healthy and diseased plants (week 6).
  • Fig. S5. Rare OTUs discriminate the initial soil microbiomes that later become associated with healthy and diseased plants.
  • Fig. S6. Ten best discriminant OTUs linked to future plant disease outcomes based on random forest analysis.
  • Fig. S7. The total abundance of Bacillus and Pseudomonas bacteria and their ability to inhibit the growth of Ralstonia solanacearum.
  • Fig. S8. Differences in bacterial community composition associated with healthy and diseased plants persisted throughout the field experiment.
  • Fig. S9. Temporal changes in discriminating rare OTUs observed in the initial microbiomes associated with healthy and diseased plants.
  • Fig. S10. Heatmaps showing the dynamics of discriminating rare OTUs associated with healthy and diseased plants during the field experiment.
  • Fig. S11. PCoA of Bray-Curtis distances of bacterial functional gene profiles in the beginning (week 0) and at the end of the field experiment (week 6).
  • Fig. S12. Comparison of bacterial community between initial bulk soil and 5-day old nylon bag samples.
  • Table S1. Physicochemical properties of initial soils that later became associated with healthy and diseased plants.
  • Table S2. Topological properties of networks associated with healthy and diseased plant microbiomes at weeks 0 and 6.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Screened OTUs enriched in initial soil microbiome associated with later healthy and diseased plants.
  • Data file S2 (Microsoft Excel format). Functional genes significantly different in initial soil microbiome associated with later healthy and diseased plants.
  • Data file S3 (Microsoft Excel format). Screened OTUs enriched in soil microbiome associated with later healthy and diseased plants at 6 week after planting.
  • Data file S4 (Microsoft Excel format). Functional genes significantly different in soil microbiome associated with later healthy and diseased plants at 6 weeks after planting.
  • Movie S1 (.mp4 format). Method of sampling middle-layer nylon bags from rhizobox.

Files in this Data Supplement: