Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Efficiency and cytotoxicity of the siRNA binding to HNTs.
  • Fig. S2. Lysosomal escape of HNTs/siRIPK4 in T24 bladder cancer cells.
  • Fig. S3. Intracellular trafficking and lysosomal escape of HNTs/siRIPK4.
  • Fig. S4. qRT-PCR of RIPK4 mRNA and Western blotting analysis of the RIPK4 protein in T24 cells after HNTs/siRIPK4 transfection.
  • Fig. S5. HNTs/siRIPK4 inhibited the growth of T24 cells in vitro by MTT assay and LDH assay.
  • Fig. S6. HNTs/siRIPK4 inhibited the growth of T24 cells in vitro (colony formation).
  • Fig. S7. Annexin V and propidium iodide staining of T24 cells treated with HNTs/siRIPK4 complexes and other formulations, and analysis of apoptosis using flow cytometry.
  • Fig. S8. After HNT-mediated RIPK4 knockdown in T24 cells, FACS was used to analyze the cell cycle.
  • Fig. S9. Tumor tissues analyzed using Western blotting from the subcutaneous xenograft model.
  • Fig. S10. Tumor tissues analyzed using IHC from the subcutaneous xenograft model.
  • Fig. S11. HNTs/siRIPK4 reduced Ki-67 expression and induced apoptosis of mice bearing T24 xenografts.
  • Fig. S12. Immunotoxicity analysis of blood of mice at 24 hours after PBS, siRIPK4, HNTs, and HNTs/siRIPK4 injection.
  • Table S1. Tumor suppression effect of different treatment on bladder weight and histopathologic changes in SD rat bladders of different groups.
  • Table S2. Biochemical parameters of blood from the mice at 24 h after last injection of various drugs.

Download PDF

Files in this Data Supplement: