Science Advances

Supplementary Materials

The PDF file includes:

  • Supplementary Methods
  • Fig. S1. Multi-alignment of RumMc radical SAM enzymes.
  • Fig. S2. Tandem mass spectra of RumC2-5 purified from cecal contents.
  • Fig. S3. Gene expression in the gut of rats monoassociated with R. gnavus E1 and heterologous expression and purification of MBP-mRumC1, mRumC1, and RumPc.
  • Fig. S4. Tandem mass spectra of N- and C-terminal fragments of RumC1 peptide present in in vivo and in vitro samples.
  • Fig. S5. Tandem mass spectra of mRumC1 mutants from which the residues involved in each thioether bridge were attributed.
  • Fig. S6. Determination of the connectivity of the thioether linkages in RumC1 by NMR.
  • Fig. S7. Cleavage of the leader N-terminal peptides of mRumC1 and anti-Cp activity assays.
  • Fig. S8. Evaluation of the ability of RumC1 to insert into bacterial lipids.
  • Fig. S9. Bacterial cytological profiling against Cp.
  • Fig. S10. Assessing RumC1 safety.
  • References (58, 59)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Theoretical and experimental spectra lists.

Files in this Data Supplement: