Science Advances

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• Fig. S1. Sequence alignment of Ego1 from different species as analyzed by ESPript 3.0 (34).
• Fig. S2. Deletion of residues 98 to 121 of Ego1 does not affect the vacuolar membrane location of Ego1 nor the subsequent vacuolar recruitment of the other EGOC components.
• Fig. S3. Strains expressing Ego1 and Ego3 mutant variants that are unable to bind the Rag GTPases are sensitive to rapamycin and have reduced TORC1 activity.
• Fig. S4. Overall structure of the two EGOC molecules in the asymmetric unit.
• Fig. S5. Representative composite 2Fo-Fc omit maps (contoured at 1σ) of the EGOC (molecule A).
• Fig. S6. Structural comparison of associated and free Gtr1-Gtr2 heterodimers.
• Fig. S7. Structural comparison of the EGOC and the Ragulator-Rag complex.
• Fig. S8. Analysis of the diffraction data with the Diffraction Anisotropy Server (26).
• Table S1. Strains used in this study.
• Table S2. Plasmids used in this study.
• References (34–38)

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