Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Depletion of hTERT leads to telomere deprotection without telomere shortening: signal-free end quantitation, meta-TIFs in a second cell line, and cell cycle quantitation.
  • Fig. S2. Reduction of telomere deprotection after overexpression of hTERT in a second cell line and for an extended period.
  • Fig. S3. Overexpression of hTERT does not alter cell cycle phase or levels of activation of the ATM DNA damage pathway.
  • Fig. S4. Genes overexpressed after hTERT expression, expression level controls, and control for hTERT antibody specificity.
  • Fig. S5. Control experiments for Fig. 3: quantitation of expression and meta-TIF levels.
  • Fig. S6. Controls for Fig. 4: antibody specificity controls and alternative data representation.
  • Fig. S7. Controls for Fig. 5: expression of TRF2 and Apollo and verification of colocalization.
  • Table S1. Silencing RNAs used in knock-down experiments.
  • Table S2. Primers used for gene-expression studies.
  • Table S3. Cycling conditions for qRT-PCR.

Download PDF

Files in this Data Supplement: