Science Advances

Supplementary Materials

The PDF file includes:

  • Sections S1 and S2. Characterization of fluorescent lipid analogs
  • Section S3. MHC-I mobility at HIV-1 assembly sites in NL4.3 Gag-iGFP HIV-1–infected Jurkat T cells
  • Section S4. STED microscope calibration
  • Section S5. PI(4,5)P2 clustering and trapping by full-length HIV-1 Gag on biomimetic membranes
  • Section S6. Budding efficiency comparison in Jurkat T cells transfected with Gag.eGFP or with a mixture of Gag.eGFP/Gag (ratio, 1:3)
  • Section S7. Cumulative frequency distributions observed in infected cells for the different lipids
  • Section S8. Cumulative frequency distributions observed in transfected cells for the different lipids
  • Fig. S1. Fluorescent lipid analog structures.
  • Fig. S2. Fluorescent lipid analog mobility in Jurkat T cells.
  • Fig. S3. MHC-I mobility at HIV-1 assembly sites in NL4.3 Gag-iGFP HIV-1–infected Jurkat T cells.
  • Fig. S4. STED microscope calibration results.
  • Fig. S5. Changes in the lateral mobility of ATTO647N-PI(4,5)P2 upon addition of Gag on SLBs.
  • Fig. S6. Jurkat T cell coelectroporation with Gag.eGFP and Gag plasmids.
  • Fig. S7. Cumulative frequency distributions of diffusion coefficient observed in HIV-1–infected T cells.
  • Fig. S8. Cumulative frequency distributions of diffusion coefficient observed in HIV-1 Gag-transfected T cells.
  • Legend for movie S1
  • References (62, 63)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Drift stabilized time lapse movie of a representative NL4.3 Gag-iGFP HIV-1–infected Jurkat T-cell showing already present and newly developing virus assembly sites.

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