Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Identification of a novel FACT-like chromatin transcription chaperone.
  • Fig. S2. In vitro transcription assays.
  • Fig. S3. Bioinformatics analysis of genes within categories defined by levels of FACT, LEDGF, and HDGF2 binding.
  • Fig. S4. HDGF2/LEDGF colocalize with H3K36me2 and moderate levels of RNAPII excluding H3K27me3 domains.
  • Fig. S5. Validation of LEDGF and HDGF2 antibodies.
  • Fig. S6. Genes in MB and MT are categorized on the basis of RNAPII occupancy.
  • Fig. S7. Highly transcribed genes with SPT16 (FACT) occupancy in MBs.
  • Fig. S8. HDGF2 is required to activate MT-specific genes.
  • Fig. S9. Genomic characterization of FACT, LEDGF, and HDGF2 in ESCs, 293T cells, MBs, and MTs.
  • Table S1. MS sequencing results for S-200 (fraction 5) as shown in Fig. 1D.
  • Table S2. Table of histone modification quantified by MS in LEDGF and HDGF2 ChIPs.
  • Table S3. Table of gRNA dropout results from CRISPR-CAS9 screens in mESCs.

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