Science Advances

Supplementary Materials

The PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Protein purification and crystallization.
  • Fig. S2. Crystal packing.
  • Fig. S3. Examples of electron density.
  • Fig. S4. Comparison of CysLT1R-pran structure and ligand binding pose with other lipid and δ-branch class A GPCRs.
  • Fig. S5. Effect of monovalent cations on CysLT1R stability.
  • Fig. S6. Effects of LTD4 and CysLT1R antagonists on IP1 production.
  • Fig. S7. Docking of LTD4 in CysLT1R.
  • Fig. S8. MD simulations of the TM4-TM5 gate closure.
  • Table S1. Crystallographic data collection and refinement statistics.
  • Table S2. Signaling and cell surface expression data for CysLT1R.
  • Legends for movies S1 to S3
  • Reference (55)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Rapid closure of the ligand access gate.
  • Movie S2 (.mp4 format). Lipid molecule enters the ligand access gate.
  • Movie S3 (.mp4 format). Spontaneous opening and closing of the ligand access gate.

Files in this Data Supplement: