Science Advances

This PDF file includes:

• Fig. S1. Simulation of the GAL pathway.
• Fig. S2. Comparison of protein expression levels of GAL genes between cis- and trans-tagging strains.
• Fig. S3. Flowchart showing the construction of cis- and trans-deletion strains for the pair of GAL1 and GAL10 genes.
• Fig. S4. Ln(cell number ratio between the cis- and trans-deletion strains) as a function of competition time.
• Table S1. Parameter values used in the simulation of the linear pathway with two reactions.
• Table S2. Parameter values used in the simulation of the GAL pathway.
• Table S3. Primers for strain construction.
• Table S4. gRNA target sequences.
• Table S5. Doubling times for various trans-deletion strains.
• References (46–51)

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