Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Tetraploid Oct4-GFP–positive cells reduce ploidy during embryo development.
  • Fig. S2. Fusion-derived cells proliferate in self-renewal conditions and originate Oct4-GFP–positive 4n and Oct4-GFP–negative 2n cells.
  • Fig. S3. Tetraploid cells undergo tripolar mitosis and form viable daughter cells.
  • Fig. S4. Parental chromosome segregation during hybrids’ division can be nonrandom.
  • Fig. S5. 4n-derived 2n cells show NPC and ESC phenotype if cultured in the respective culture medium.
  • Legends for movies S1 to S21
  • Legend for table S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Time-lapse imaging of an embryo injected with one single PB-dsRED 4n cell. Example 1.
  • Movie S2 (.avi format). Time-lapse imaging of an embryo injected with one single PB-dsRED 4n cell. Example 2.
  • Movie S3 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 1.
  • Movie S4 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 2.
  • Movie S5 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 3.
  • Movie S6 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 4.
  • Movie S7 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 5.
  • Movie S8 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 6.
  • Movie S9 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 7.
  • Movie S10 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 8.
  • Movie S11 (.avi format). Fly-through images of CT studies performed in 8-week-old chimeric mouse. Mouse 9.
  • Movie S12 (.avi format). Time-lapse images of a synkaryon 4n cell carrying mRFP-tagged histone H2B (H2B-RFP) undergoing bipolar division.
  • Movie S13 (.avi format). Time-lapse images of a synkaryon 4n cell carrying H2B-RFP undergoing tripolar division.
  • Movie S14 (.avi format). Time-lapse images of a synkaryon 4n cell carrying H2B-RFP undergoing tripolar division without mitotic catastrophe.
  • Movie S15 (.avi format). Time-lapse images of a sorted hybrid cell generated after fusion between ESC-H2B-eGFP and NPC-H2B-mRFP that does not undergo mitosis.
  • Movie S16 (.avi format). Time-lapse images of a sorted hybrid cell generated after fusion between ESC-H2B-eGFP and NPC-H2B-mRFP that undergoes bipolar mitosis.
  • Movie S17 (.avi format). Time-lapse images of a sorted hybrid cell generated after fusion between ESC-H2B-eGFP and NPC-H2B-mRFP that undergoes tripolar mitosis with random segregation.
  • Movie S18 (.avi format). Time-lapse images of a sorted hybrid cell generated after fusion between ESC-H2B-eGFP and NPC-H2B-mRFP that undergoes tripolar mitosis with non-random segregation.
  • Movie S19 (.avi format). Time-lapse images of a sorted hybrid cell generated after fusion between ESC-H2B-eGFP and NPC-H2B-mRFP with parental chromosomes showing different spatial occupancy after bipolar mitosis.
  • Movie S20 (.avi format). Time-lapse images of a sorted hybrid cell generated after fusion between ESC-H2B-eGFP and NPC-H2B-mRFP with parental chromosomes showing different spatial occupancy after tripolar mitosis.
  • Movie S21 (.avi format). Time-lapse images of a long-time tracking a sorted hybrid cell generated after fusion between ESC-H2B-eGFP and NPC-H2B-mRFP.
  • Table S1 (Microsoft Excel format). SNP genotyping raw data.

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