Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Global analysis of WGBS data.
  • Fig. S2. GO analysis of genes associated with hypomethylated DMRs upstream of the TSS and downstream of the TES.
  • Fig. S3. Genes with intragenic hypomethylated DMRs preferentially become activated in neurons.
  • Fig. S4. Many intragenic hypomethylated DMRs are NEUROD2 targets.
  • Fig. S5. Accumulation of 5hmC at NEUROD2 binding sites undergoing DNA demethylation.
  • Fig. S6. Luciferase assays with methylated and unmethylated NEUROD2-targeted regions.
  • Fig. S7. DNA methylation changes at NEUROD2 target genes in Neurod2 knockout mice.
  • Fig. S8. Analysis of NEUROD2 target genes in P19 cells.
  • Fig. S9. Analysis of NEUROD2 target genes in P19 cells after Tet2 knockdown.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). DMRs between neurons and NPCs identified by the DSS method.
  • Table S2 (Microsoft Excel format). Differential expression of genes with intragenic hypomethylated DMRs.
  • Table S3 (Microsoft Excel format). NEUROD2 peaks at H3K27Ac-enriched hypomethylated DMRs within genes.
  • Table S4 (Microsoft Excel format). Primers for bisulfite sequencing and RT-PCR.

Files in this Data Supplement: