Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Screening of sgRNAs targeting the Rpe65 gene in rd12 MEFs.
  • Fig. S2. Delivered AAV copies between retina and RPE.
  • Fig. S3. Analysis of human RPE65 editing.
  • Fig. S4. Electroretinographic responses to bright stimuli (0 dB) after dark adaptation from normal C57BL/6 and rd12 mice at 6 weeks after the injection (n = 4).
  • Fig. S5. Electroretinographic responses to dim stimuli (−25 dB) after dark adaptation from normal C57BL/6 and rd12 mice at 6 weeks after the injection.
  • Fig. S6. Analysis of TS4 sgRNA off-target effects.
  • Fig. S7. Comparison of off-target frequency between wild-type SpCas9 and SniperCas9.
  • Table S1. Summary of predictions at the target site with sgRNA by the inDelphi approach.
  • Table S2. Active off-target sites.
  • Table S3. Primers used in this study.

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