Science Advances

Supplementary Materials

The PDFset includes:

  • Fig. S1. Characterization of APEX2F-OPTN recruitment to depolarized mitochondria.
  • Fig. S2. Analysis of proximity biotinylation using APEX2F-OPTN and APEX2F-TAX1BP1 in response to mitochondrial depolarization.
  • Fig. S3. Benchmarking and validation of an mt-Keima–based screening platform for CRISPR-Cas9 analysis of mitophagic flux.
  • Fig. S4. The HK2 requirement for PINK1 activation is not due to ATP depletion and is also observed in H9 hES.

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Other Supplementary Material for this manuscript includes the following:

  • Dataset S1 (Microsoft Excel format). Tables containing proteomic identification of proteins in proximity to APEX2-OPTN at 1 and 3 hours after depolarization determined in duplicate.
  • Dataset S2 (Microsoft Excel format). Tables containing proteomic identification of proteins in proximity to APEX2-OPTN at 1 hour after depolarization in triplicate.
  • Dataset S3 (Microsoft Excel format). Tables containing proteomic identification of proteins in proximity to APEX2-OPTND474N at 1 hour after depolarization in triplicate.
  • Dataset S4 (Microsoft Excel format). Tables containing proteomic identification of proteins in proximity to APEX2-TAX1BP1 at 1 hour after depolarization in triplicate.
  • Dataset S5 (Microsoft Excel format). Tables containing target sgRNA sequences used to create custom CRISPR libraries, as well as raw sequence reads and MAGeCK scores from the mitophagic flux screens performed using mt-Keima flux assays.

Files in this Data Supplement: