Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Effects of gain and loss of EGFR signaling on sleep architecture.
  • Fig. S2. Amino acid alignment of human and zebrafish TGFa, EGF, and EGFR.
  • Fig. S3. Gefitinib does not enhance egfra−/− phenotype and effects of EGFR inhibitors on sleep architecture.
  • Fig. S4. EGFR signaling is not required for behavioral circadian rhythms.
  • Fig. S5. Validation of an SD assay, and EGFR signaling is required for normal homeostatic regulation of sleep.
  • Fig. S6. Inhibition of MAPK/ERK signaling suppresses TGFa overexpression–induced sleep.
  • Fig. S7. EGFR signaling regulates npvf expression, and TGFa overexpression–induced sleep is suppressed in npvf mutant animals.
  • Fig. S8. Association of ERBB4 sleepiness allele with increased ERBB4 expression in humans and pharmacological inhibition of KSR2 or ERBB4 decrease sleep in zebrafish.
  • Table S1. Variants at ERBB4 and KSR2 associate with self-reported measures of sleep quality and quantity in U.K. Biobank subjects.
  • Table S2. Descriptive characteristics of U.K. Biobank subjects of European ancestry used for sleep trait analysis.

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