Science Advances

Supplementary Materials

The PDFset includes:

  • Fig. S1. No mitochondrial transfer from conditioned medium to MLO-Y4 cells.
  • Fig. S2. Osteocytes do not pass through the pores of the transwell membrane.
  • Fig. S3. Depleted mtDNA and impeded ATP production in MLO-Y4 ρ° cells.
  • Fig. S4. Parental MLO-Y4 cells and primary osteocytes can transfer mitochondria to MLO-Y4 ρ° cells.
  • Fig. S5. Mitochondrial distribution and ER movement in osteocyte dendrites.
  • Fig. S6. Knockdown of Mfn2 has no effect on ATP production and the level of mtDNA.
  • Fig. S7. Mfn2 knockdown impedes mitochondrial transfer in MLO-Y4 cells.
  • Fig. S8. VAPB knockdown impedes mitochondrial transfer in MLO-Y4 cells.
  • Fig. S9. Hypothetical model of ER-mediated mitochondrial transfer.
  • Table S1. Primer sequences for qPCR.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). pTaqYFP-mito–labeled mitochondria (green) in the dendrite dynamically migrate toward the PKH26-stained cells (magenta).
  • Movie S2 (.mp4 format). pTaqYFP-mito–labeled mitochondria (green) dynamically move from the cytoplasm to the dendritic process and are associated with RFP-labeled ER (magenta).
  • Movie S3 (.mp4 format). Transfer of MTR-labeled mitochondria (magenta) within dendrites is dynamically colocalized with GFP-labeled ER (green).
  • Movie S4 (.mp4 format). Transfer of MTR-labeled mitochondria (magenta) is dynamically colocalized with YFP-labeled Mfn2 (green) within dendrites.

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