Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Size distribution and DNA analysis of pancreatic islet spheroids over 4 weeks of culture.
  • Fig. S2. Immunofluorescent detection of iECs on flow chips.
  • Fig. S3. Simulation of flow velocity and shear stress on channel bottom and spheroid surface.
  • Fig. S4. Interstitial flow effect on expansion of iECs cultured in conditioned media.
  • Fig. S5. Simulation of the diffusion and consumption of nutrients introduced into the culture systems.
  • Fig. S6. Fast flow condition beyond interstitial levels resulted in decreased viability of islet spheroids.
  • Fig. S7. Simulation of localized accumulation of secreted soluble factors from islet spheroids under two different dynamic conditions.
  • Fig. S8. Long-term culture (4 weeks) of islet spheroids in microfluidic chips.
  • Table S1. Primer design for qRT-PCR.
  • References (4151)

Download PDF

Files in this Data Supplement: