Science Advances

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• Fig. S1. Automatic detection, filtering, and quantification of CCP dynamics from TIRF movies.
• Fig. S2. FCHo2 arrives as early as clathrin is linearly recruited and leaves CCPs before scission.
• Fig. S3. Impaired Tf-CME in FCHo2-depleted HEK293T and HeLa cells.
• Fig. S4. EM analysis of clathrin-coated endocytic structures following lentiviral knockdown of FCHo2 protein expression.
• Fig. S5. Expression of HA-tagged FCHo2 mutants, deletions, and chimeras.
• Fig. S6. Limited diffusion of plasma membrane Harvey rat sarcoma viral oncogene homolog (hRas)–SNAP into the center of CCPs delineated by eGFP-FCHo2.
• Table S1. Interaction energies between the different proteins and the proteins and the membrane, in units of k_BT.

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