Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. PRM detection and quantification limit.
  • Fig. S2. Collision energy optimization and Skyline implementation into the pipeline.
  • Fig. S3. Representative MS2 spectra of IgG N-glycopeptides from SILAC-PRM analysis.
  • Fig. S4. Data prediction accuracy by the model: intracellular IgG N-glycan distribution.
  • Fig. S5. Data prediction accuracy by the model: secreted IgG N-glycan distribution.
  • Fig. S6. Effects of different perturbations on N-glycan processing.
  • Fig. S7. Man4GlcNAc2 generation.
  • Fig. S8. Kinetic mechanisms considered for Man4GlcNAc2 formation.
  • Table S1. List of glycans selected for PRM analysis of glycopeptides.
  • Table S2. ER-related parameters.

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