Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Synthesis and characterization of carboxylated branched PBAE polymers.
  • Fig. S2. Synthesis and characterization of carboxylate ligands.
  • Fig. S3. Cell viability after treatment with carboxylated branched PBAE protein nanoparticles.
  • Fig. S4. Confocal images of cells treated with C5/FITC-BSA nanoparticles.
  • Fig. S5. Characterization of polymer pH buffering and endosomal disruption capabilities.
  • Fig. S6. C5/RNP nanoparticles enable in vitro gene deletion.
  • Fig. S7. C5/RNP nanoparticles are stable in serum-containing media and in lyophilized form.
  • Fig. S8. C5/RNP nanoparticle-enabled in vivo CRISPR editing is reproducible.
  • Table S1. Characteristics of proteins and encapsulated C5 nanoparticles and optimal nanoparticle formulations used in this study.
  • Table S2. DNA sequences.

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