Science Advances

Supplementary Materials

The PDFset includes:

  • Supplementary Materials and Methods
  • Fig. S1. Structure determination of wild-type complex I, assembly intermediate, and mutant without NDUFS4.
  • Fig. S2. Representative cryo-EM densities of wild-type complex I with fitted models.
  • Fig. S3. Assignment of accessory subunits.
  • Fig. S4. A second Q binding site in complex I and quantitative mass spectrometry.
  • Fig. S5. Atomic model of Y. lipolytica complex I in membrane-solvent environment and hydrogen bond analysis from MD simulation trajectory.
  • Fig. S6. Interactions between protein and the Q head group and tail.
  • Fig. S7. Lipid binding sites in yeast and mammalian complex I.
  • Fig. S8. Surface helices and lipids modeled at the interface of the membrane arm and the peripheral arm.
  • Fig. S9. Bending of the lipid tails seen in MD simulation.
  • Fig. S10. Spectrophotometric assay of NADH:DBQ activity.
  • Fig. S11. Conformation of the β1-β2 loop of NDUFS2 and loops and regions critical for the active/deactive transition.
  • Fig. S12. Proton pumping activity of the NDUFS4 mutant.
  • Fig. S13. Cartoon representation and sequence alignment of NDUFAF2 and NDUFA12 from Y. lipolytica and Homo sapiens.
  • Table S1. Data collection, refinement, and model statistics.
  • Table S2. Subunit names and chain identifiers for Y. lipolytica and human and bovine complex I.
  • Table S3. Lipid species detected in Y. lipolytica complex I.
  • Legends for movies S1 and S3
  • References (7378)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Cryo-EM structure of respiratory complex I from Y. lipolytica in LMNG at 3.2-Å resolution.
  • Movie S2 (.mp4 format). Movie S2. Cryo-EM structure of ΔNDUFS4 at 4.0-Å resolution.
  • Movie S3 (.mp4 format). Movie S3. Cryo-EM structure of the ΔNDUFS6 assembly intermediate at 3.3-Å resolution.

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