Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. MBA design, layout, and operation.
  • Fig. S2. Initial bioreactor seeding with PAX2+ cell population.
  • Fig. S3. The day 9 end point MBA.
  • Fig. S4. The process flow for the MBA image cytometry.
  • Fig. S5. Pareto chart of the standard effects and the main effects plots from a full factorial design of experiments multivariate analysis day 9 MBAs.
  • Fig. S6. Analysis of the effect of continued Wnt signaling and RA activity after variation in the initial duration of canonical Wnt patterning (3 days initial CHIR).
  • Fig. S7. Image cytometry comparison for the first row of the day 3 to 8 and day 4 to 9 MBAs.
  • Fig. S8. Pareto chart of the standard effects and the main effects plots from a full factorial design of experiments multivariate analysis day 3 to 8 MBAs.
  • Fig. S9. Heat maps of cell distributions with all MBA sets (day 9, day 12, days 3 to 8, and days 4 to 9).
  • Fig. S10. Investigating the paracrine and autocrine effect in the MBAs.

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