Science Advances

Supplementary Materials

The PDFset includes:

  • Fig. S1. Distribution of simulated r coefficients for the correlation between undernutrition and microcephaly cases in tropical states of Brazil obtained after permutation of the original dataset.
  • Fig. S2. Number of ZIKV infection confirmed cases in each Brazilian state.
  • Fig. S3. Viral load in E18 placentas was quantified by RT-qPCR.
  • Fig. S4. Confirmation of ZIKV detection by immunohistochemistry in E15.
  • Fig. S5. Viral replication in LP/ZIKV placentas at E15.
  • Fig. S6. H&E staining in E15 placentas.
  • Fig. S7. Viral load in E15 and E18 embryonic brains quantified by RT-qPCR.
  • Fig. S8. ZIKV-infected cells colocalize with endothelial cells in E15 brains.
  • Fig. S9. ZIKV-infected cells colocalize with microglial cells in E15 brains.
  • Fig. S10. Staining of J2 on E15 embryonic brains colocalized with IB4.
  • Fig. S11. Network describing altered genes involved in the mTOR pathway.
  • Fig. S12. Morphometric analysis of brain images obtained through ╬╝CT.
  • Fig. S13. Skull size of LP/ZIKV newborns is reduced.
  • Fig. S14. Summary of the results obtained with the experimental model.
  • Fig. S15. Validation of differentially expressed genes by RT-qPCR in E15 brains of Co/ZIKV and LP/ZIKV.
  • Table S1. Relationship between cases of undernutrition reported in hospitals and medical services and cases of microcephaly between 2015 and 2018.
  • Table S2. The estimated amount of daily protein (g) intake for each of the 83 interviewed mothers who have children with CZS.
  • Table S3. Relative risk of CZS in relation to protein intake.
  • Table S4. Maternal body weight (mean and SD) at the different pregnancy stages of the mouse model.

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Table S5 (Microsoft Excel format). Differentially expressed genes between Co/ZIKV and LP/ZIKV.

Files in this Data Supplement: