Science Advances

Supplementary Materials

The PDFset includes:

  • Fig. S1. Functional characterization of PRDM15 mutations associated with SRNS and microcephaly/HPE.
  • Fig. S2. Constitutive and conditional Prdm15 KO alleles.
  • Fig. S3. Sox2-Cre epiblast-specific deletion of Prdm15 recapitulates the Prdm15Lacz phenotype.
  • Fig. S4. Identification of PRDM15 transcriptional targets in the pre-gastrula mouse embryo.
  • Fig. S5. CRISPR-Cas9–mediated knock-in of the mouse C842 mutation in ESCs.
  • Fig. S6. mC842Y/hC844Y substitution is an LOF mutation.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). ChIP-sequencing and RNA-sequencing analysis.
  • Table S2 (Microsoft Excel format). Genetic variants of PRDM15 target genes associated with HPE.
  • Table S3 (Microsoft Excel format). Oligonucleotides used for site-directed mutagenesis and mutation knock-in.
  • Table S4 (Microsoft Excel format). qRT-PCR primers and ChIP-qPCR primers.

Files in this Data Supplement: