Science Advances

Supplementary Materials

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  • Fig. S1. Comparison of Fn14 mRNA expression levels in normal breast versus breast cancer subtypes and Fn14 expression/breast cancer patient survival analysis.
  • Fig. S2. Analysis of Fn14-specific binding of free targeting ligands and corresponding ligand-conjugated nanoparticles using SPR assays.
  • Fig. S3. Surface properties of nanoparticles affect blood clearance time and liver accumulation following intravenous injection.
  • Fig. S4. Analysis of Fn14 expression using Western blot and fluorescence-activated cell sorting analyses.
  • Fig. S5. SPR analysis reveals Fn14-targeted nanoparticles maintain specific binding interactions with Fn14 even after incubation with mouse blood serum.
  • Fig. S6. Effect of PTX and Abraxane on MB-231-Luc cell viability.
  • Fig. S7. Nontargeted and Fn14-targeted DART nanoparticles do not exhibit nonspecific interaction with tumor ECM proteins as determined using SPR analysis.
  • Fig. S8. PTX-loaded DART nanoparticles inhibit 231-Luc tumor growth after intratumoral injection.
  • Fig. S9. PTX-loaded, Fn14-targeted DART nanoparticles do not induce histologic evidence of inflammatory or cytotoxic damage to healthy tissues.
  • Fig. S10. Effect of PTX on MB-231-Br-Luc cell viability and distribution of PLGA-PEG-IgG and PLGA-PEG-ITEM4 nanoparticles after systemic administration into mice bearing TNBC tumors in the brain.

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