Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Binding and thermo-stabilizing effect of JN241 to APJ and formation of stable APJ-JN241 complex and co-crystals.
  • Fig. S2. Simple omit maps of JN241 CDR1, CDR2 and CDR3.
  • Fig. S3. Characterization of JN241 epitope and identification of the critical residue E174 in APJ ECL2 for its binding and function.
  • Fig. S4. Amino acid sequence alignment of JN241 and its nine mutants.
  • Fig. S5. Comparison of the receptor-ligand interactions.
  • Table S1. Data collection and structure refinement statistics.
  • Table S2. Interaction residues on APJ-JN241 interface according to the cocrystal structure.
  • Table S3. EC50 and IC50 values of JN241 and its mutants fused to human Fc in APJ cAMP and β-arrestin assays.
  • Table S4. Conservation of WT APJ, AT1R, and AT2R residues critical for ligand binding.

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