Science Advances

Supplementary Materials

The PDFset includes:

  • Supplementary Materials and Methods
  • Fig. S1. Photoablation-guided fabrication of acellular capillary-sized microchannels.
  • Fig. S2. Cellular limitations of capillary fabrication.
  • Fig. S3. Endothelialization strategies for capillary network.
  • Fig. S4. Ultrastructural analysis of vessels at capillary regions across the ACV units imaged via TEM.
  • Fig. S5. Edge detection workflow for studying traversal of single RBCs through a capillary-sized constriction.
  • Fig. S6. Transcriptional profiling of var gene expression in the two parasite lines.
  • Fig. S7. Ultrastructural analysis of capillary region after perfusion of RBCs imaged via TEM.
  • Fig. S8. Trypsin cleaves the surface-expressed IT4VAR19 PfEMP1 variant.
  • Legends for movies S1 to S10
  • References (61, 62)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Endothelial ingrowth into 20-μm capillaries between two large vessels after photoablation.
  • Movie S2 (.mp4 format). Representative video of normal RBCs perfused through endothelialized capillaries at physiological hematocrit.
  • Movie S3 (.mp4 format). Representative video of normal RBCs perfused through acellular collagen capillary-shaped channels at physiological hematocrit accumulating a significant amount before entering the capillary region and jetting and leading to significantly lower hematocrit into the downstream.
  • Movie S4 (.mp4 format). Representative video of normal RBCs stretching through capillaries (diameter, ~10 μm) when perfused at the single-cell scale.
  • Movie S5 (.mp4 format). Representative video of normal RBCs tumbling through capillaries (diameter, ~10 μm) when perfused at the single-cell scale.
  • Movie S6 (.mp4 format). Representative video of 2G2 IRBCs tumbling through capillaries (diameter, ~10 μm) when perfused at the single-cell scale.
  • Movie S7 (.mp4 format). Representative video of normal RBCs perfused through the capillaries at physiological hematocrit with no cell accumulation in the lumen.
  • Movie S8 (.mp4 format). Representative video of IT4VAR19 IRBCs perfused through the capillaries at physiological hematocrit accumulating a significant amount and blocking the capillary flow.
  • Movie S9 (.mp4 format). Representative video of trypsinized IT4VAR19 perfused through the capillaries showing a significant decrease in cell accumulation and not blocking the capillary flow.
  • Movie S10 (.mp4 format). Representative video of 2G2 IRBCs perfused through the capillaries at physiological hematocrit accumulating at postcapillary regions but not blocking the capillary flow.

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