Science Advances

Supplementary Materials

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  • Fig. S1. Site locations for the 180 unique soil samples for which we obtained 18S rRNA gene sequence data (green circles; 137 samples for which we also obtained 16S rRNA gene amplicon data) and 46 samples for which we obtained SSU rRNA gene data from shotgun metagenomic sequencing (blue circles).
  • Fig. S2. Pairwise correlation heatmap for the climatic and edaphic characteristics associated with the 180 soils analyzed.
  • Fig. S3. Detection of the top 50 dominant protistan lineages by 18S rRNA amplicon sequencing versus shotgun metagenomic sequencing.
  • Fig. S4. Factors shaping protistan community (namely, climatic variables) are distinct from those factors shaping prokaryotic communities (namely, pH).
  • Fig. S5. The top predictors of ubiquitous protist lineages (protistan ASVs were phylogenetically clustered into clades at 96% similarity).
  • Fig. S6. A heatmap of the correlation coefficients based on semipartial correlations between the relative abundance of protistan clades (one clade per row) that had significant semipartial correlations to environmental predictors (n = 43 of 50 protist clades that were predictable in distribution).
  • Table S1. Location and site information for the 180 soils for which we obtained 18S rRNA gene amplicon data.
  • Table S2. Information on the 116 abundant protistan lineages (phylogenetic clades) including taxonomic affiliation, the percent variance explained (var.expl), the top environmental predictor from random forest models (predictor1), the number of ASVs in the lineage (freq.), and the ecological cluster affiliation.
  • Table S3. Location and site information for the 46 soils for which we obtained shotgun metagenomic data.

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