Science Advances

Supplementary Materials

This PDF file includes:

  • Section S1. Materials
  • Section S2. Fabrication of PDMS-based microfluidic chips
  • Section S3. Theory and numerical simulation
  • Section S4. Microfluidic flow synthesis of enzyme-MOF composites
  • Section S5. Bulk solution synthesis of enzyme-MOF composites
  • Section S6. Zeta-sizer analysis of Cyt c–MOF composites
  • Section S7. TEM and EDS analysis
  • Section S8. Powder XRD of enzyme-MOFs and pattern analysis
  • Section S9. SEM analysis of pure MOFs and enzyme-MOF composites
  • Section S10. Measurements of the loading amount of enzymes in MOFs
  • Section S11. Determination of the activity of enzyme-MOF composites
  • Section S12. XAFS spectra of bulk solution and microfluidic flow–synthesized ZIF-8 crystal
  • Section S13. Bulk solution synthesis of enzyme-MOF composites of varied reactants ratio and characterization results in contrast with microfluidic flow synthesis
  • Section S14. Investigation of the effect of flow rate on activity of enzyme-MOF composites
  • Section S15. Spatially controlled synthesis of Au NP–MOF composites
  • Section S16. Stability of enzyme-MOF composites at high temperature and in the presence of protease digestion
  • Section S17. Recyclability and long-term stability of enzyme-MOFs
  • Section S18. The influence of flow rate on defect properties
  • Section S19. Numerical calculation of substrate diffusion in enzyme-MOFs
  • Fig. S1. Pictures of the microfluidic chip for synthesis of enzyme-MOF composites.
  • Fig. S2. Numerical simulation of velocity distribution in the microchannel.
  • Fig. S3. Numerical simulation results of concentration distribution of reactants in different positions in the microchannel.
  • Fig. S4. Three on-chip mixing schemes and corresponding simulation results of laminar flow diffusive mixing for the synthesis of enzyme-MOF composites.
  • Fig. S5. Simulation results of laminar flow diffusive mixing for the synthesis of enzyme-MOF composites using mixing order scheme 1 with tuned flow rates.
  • Fig. S6. The real image of the setup for microfluidic flow synthesis of enzyme-MOF composites.
  • Fig. S7. Zetasizer analysis of Cyt c–MOF composites prepared by microfluidic synthesis with scheme 1.
  • Fig. S8. TEM and EDS mapping images of Cyt c–MOF composites prepared on microfluidic chips with different mixing schemes.
  • Fig. S9. XRD patterns of pure MOFs and the Cyt c–MOF composites obtained using scheme 1 on microfluidic chip with the indexation of peaks.
  • Fig. S10. SEM images of pure MOFs and enzyme-MOF composites.
  • Fig. S11. TGA curves of GOx- and HRP-MOF composites in air.
  • Fig. S12. Relative enzyme activity of Cyt c–MOF composites prepared by microfluidic synthesis with different mixing schemes.
  • Fig. S13. Relative enzyme activity of different enzymes before and after lyophilization process.
  • Fig. S14. Comparison of catalytic activity between different constituents of enzyme-MOF composites and their combination.
  • Fig. S15. EXAFS spectra and feff fit curve of bulk solution–synthesized and microchannel-synthesized MOFs.
  • Fig. S16. N2 adsorption-desorption isotherm of the MOF particles prepared by microfluidic synthesis (scheme 1) and bulk solution synthesis with different metal/ligand ratios.
  • Fig. S17. Investigation of the effect of flow rate on activity of HRP-MOF composites.
  • Fig. S18. HAADF-STEM images of Au NP–MOF composites prepared by bulk solution synthesis and microfluidic flow synthesis.
  • Fig. S19. The long-term stability of microfluidic and bulk solution synthesized HRP-MOF composites.
  • Fig. S20. The reusability of HRP-adsorbed MOFs and microfluidic- and bulk solution–synthesized HRP-MOF composites.
  • Fig. S21. Pore size distributions of MOF particles calculated using DFT method.
  • Fig. S22. Schematic figure of the size of substrate and pore size of enzyme-MOF composites prepared by microfluidic synthesis and bulk solution synthesis.
  • Fig. S23. Numerical simulation result of substrate diffusion into enzyme-MOF composites.
  • Table S1. Literature data of diffusion coefficient.
  • Table S2. Data obtained from fitting XAFS spectra to pure ZIF-8 crystal feff path.
  • Table S3. Data of ICP-OES and elemental analysis of MOFs prepared by bulk solution synthesis and microfluidic synthesis (scheme 1).
  • Table S4. Pore volume data of MOFs synthesized by bulk solution synthesis and microfluidic synthesis (scheme 1).
  • References (4958)

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