Science Advances

Supplementary Materials

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  • SUPPLEMENTAL STRAIN CONSTRUCTION METHODS
  • Fig. S1. Schematic of the microfluidic device.
  • Fig. S2. Flagellar gene expression is heterogeneous when cells are grown in liquid suspension.
  • Fig. S3. Flow cytometry analysis of flagellar gene expression for cells grown in liquid culture.
  • Fig. S4. The cis-YFP reporter in the FlhDC operon does not affect class II gene expression.
  • Fig. S5. Estimation of promoter activity from time-lapse data.
  • Fig. S6. Class I (flhDp) promoter activity resembles a constitutive promoter.
  • Fig. S7. Promoters within the same class show high correlation.
  • Fig. S8. Calibration of class I reporter for mut4 and T7 RBS.
  • Fig. S9. An insertion element mutation in the FlhDC regulatory region results in high homogenous expression of flagellar genes.
  • Fig. S10. Effect of flagellar gene deletions on class II pulses.
  • Fig. S11. Constitutively expressed YdiV can restore class II promoter pulses in a ∆ydiV mutant of E. coli.
  • Fig. S12. Comparison of Salmonella and E. coli class II and III gene expression.
  • Table 1. List of plasmids.
  • Table 2. List of strains.
  • Table 3. List of the combination of promoter and RBS used to control class I expression and the notation used in this work to reference them.
  • References (6676)

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