Science Advances

The PDFset includes:

• Fig. S1. Absorption spectra of an NM-R3 crystal before and after laser irradiation.
• Fig. S2. Comparison of retinal conformations before and after laser irradiation.
• Fig. S3. Entire difference Fourier electron density map and average temperature factors of NM-R3 crystal structure in the dark and light state under different laser irradiation conditions.
• Fig. S4. Stereoscopic views of the difference electron density maps near the retinal.
• Fig. S5. Pathway for chloride ion transfer from extracellular to cytoplasmic face.
• Fig. S6. Difference distance matrix plots showing differences between the dark and light states of NM-R3.
• Fig. S7. Comparison of the NM-R3, HsBR, and HsHR retinal-binding pockets.
• Fig. S8. Mutation analysis of Ser²³⁴, an important residue for NM-R3 function.
• Table S1. Data collection and refinement statistics.
• Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Detailed view of changes around the chromophore of NM-R3.
• Movie S2 (.mp4 format). Difference electron density map showing changes at the chromophore of NM-R3.