Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Material and Methods
  • Fig. S1. Evaluation of in vivo S100a8 crRNA cleaving efficiency in mouse embryos.
  • Fig. S2. PCR analysis of genomic DNA from F0 founder number 6 after HTTP integration in the S100a8 locus at different touch down/annealing temperature conditions using primer pair (d4/r4) (Fig. 1D).
  • Fig. S3. Sequence analysis of heterozygous animal (F1) number 45 with MC head to tail integration of the DNA template in the S100a8 gene (Figs. 1E and 2A).
  • Fig. S4. Analysis of the Inf2 targeted locus.
  • Fig. S5. Analysis of the Trek1 targeted locus.
  • Fig. S6. Analysis of the Trpc6 targeted locus.
  • Fig. S7. Analysis of the Ccnd2 targeted locus.
  • Table S1. List of crRNAs used.
  • Table S2. Designed donor DNA templates.
  • Table S3. List of oligonucleotides used for ssDNA donor template generation by asymmetric PCR and PCR analyses of targeted loci.

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